Research

We aim to understand cross-species mechanisms by which genetic programs and postnatal environment regulate brain development and dysfunction at different levels. To do this, we use state-of-art molecular, cellular, circuit, physiological, anatomical and behavioral approaches in mouse and non-human primates. Our recent work demonstrated that visual experience is required for both the functional and cell-type specification of L2/3 glutamatergic neurons in the mouse primary visual cortex (Tan et al., Neuron 2020, Current Biology 2021, J. Neurosci. 2022; Cheng, Butrus and Tan et al., Cell 2022). We are building up and expanding our research topics from these findings, to ask how postnatal environment regulates the development of cell-type specific circuits at whole brain level. In addition, we are trying to rebuild functional circuits in animal models of nervous system injury. The ultimate goal of our research is to treat brain dysfunction in human.

我们实验室研究遗传程序和出生后的环境在不同水平上调控大脑发育和功能障碍的跨物种机制。 为此，我们在小鼠和非人类灵长类动物中使用最先进的分子、细胞、环路、生理学、解剖学和行为学方法。 我们最近的工作表明，小鼠初级视觉皮层中 L2/3 谷氨酸能神经元的功能和细胞亚型确立都需要视觉经验（Tan et al., Neuron 2020, Current Biology 2021, J. Neurosci. 2022; Cheng, Butrus and Tan et al., Cell 2022) 。 我们正在根据这些发现建立和扩展我们的研究课题，以探究后天环境如何在全脑水平上调节由特定细胞亚型构成的环路的发育。此外，我们正尝试在神经系统损伤的动物模型中重建功能环路。 我们研究的最终目标是治疗人类的脑功能障碍。

Biography

2022-present, Principal investigator at SIAT CAS.

2016-2022, Postdoctoral Fellow at University of California, Los Angeles, co-advised by Profs. Joshua T. Trachtenberg, S. Lawrence Zipursky and Dario L. Ringach to investigate how early life experience regulate the assembly and plasticity of cortical circuitry at molecular, cellular, circuit and systems levels.

2010-2016, PhD degree in Department of Biological Chemistry, University of California, Los Angeles with Prof. S. Lawrence Zipursky, and identified determinants of synaptic specificity in Drosophila through molecular and multi-omics approaches

2006-2010, Zhejiang University, BSc in Chemistry.

2022-今，中科院深圳先进院，研究员

2016-2022，加州大学洛杉矶分校，博士后，和Joshua T. Trachtenberg, S. Lawrence Zipursky, Dario L. Ringach教授合作，研究出生后经验如何在分子、细胞、环路和系统水平上调控视皮层环路的组装和可塑性。

2010-2016，加州大学洛杉矶分校，生物化学系，博士，在S. Lawrence Zipursky教授的指导下，在果蝇里发现了决定突触形成特异性的新型分子机制。

2006-2010，浙江大学化学系，学士

Selected publications

 (# Equal contribution, * Co-corresponding)

1. Tan L, Ringach DL, Trachtenberg JT*. The development of receptive field tuning properties in mouse binocular primary visual cortex. Journal of Neuroscience. 2022. 42(17): 3546-3556.

2. Cheng S^#, Butrus S^#, Tan L^#, Xu R, Sagireddy S, Trachtenberg JT, Shekhar K*, Zipursky SL*. Vision-dependent specification of cell types and function in the developing cortex. Cell. 2022. 185(2):311-327. e24.

3. Tan L, Ringach DL, Zipursky SL, Trachtenberg JT*. Vision is required for the formation of binocular neurons prior to the classical critical period. Current Biology. 2021. 31: 4305-4313.

4. Tan L, Tring E, Ringach DL, Zipursky SL, Trachtenberg JT*. Vision changes the cellular    composition of binocular circuitry during the critical period. Neuron. 2020. 108(4):735-747. e6.

5. Xu C, Theisen E, Maloney R, Peng J, Santiago I, Yapp C, Werkhoven Z, Rumbaut E, Shum B,  Tarnogorska D, Borycz JA, Tan L, Courgeon M, Griffin T, Levin R, Meinertzhagen IA, de Bivort B, Drugowitsch J, Pecot MY*. Control of Synaptic Specificity by Establishing a Relative Preference for Synaptic Partners. Neuron. 2019. 103(5): 865-877. e7

6. Ashley J, Sorrentino V, Nagarkar-Jaiswal J, Tan L, Xu S, Xiao Q, Zinn K*, Carrillo RA*. Transsynaptic interaction between IgSF proteins DIP-α and Dpr10 are required for motor neuron targeting specificity. eLife. 2019. 8, e42690.

7. Venkatasubramanian L, Guo Z, Xu S, Tan L, Xiao Q, Nagarkar-Jaiswal S, Mann RS*. Stereotyped Terminal Axon Branching of Leg Motor Neurons Mediated by IgSF Proteins DIP-α and Dpr10. eLife. 2019. 8, e42692.

8. Xu S^#, Xiao Q^#, Cosmanescu F, Sergeeva AP, Yoo J, Lin Y, Katsamba PS, Ahlsen G, Kaufman J, Linaval N, Lee P-T, Bellen HJ, Shapiro L, Honig B, Tan L*, Zipursky SL*. Interactions between the Ig-Superfamily Proteins DIP-α and Dpr6/10 Regulate Assembly of Neural Circuits. Neuron. 2018. 100(6):1369-1384. e6. (Previewed in the same issue)

9. Cosmanescu F, Katsamba PS, Sergeeva AP, Ahlsen G, Patel SD, Brewer JJ, Tan L, Xu S, Xiao Q, Nagarkar-Jaiswal S, Nern A, Bellen HJ, Zipursky SL*, Honig B*, and Shapiro L*. Neuron Sub-type Specific Expression, Interaction Affinities, and Specificity Determinants of DIP/Dpr Cell Recognition Proteins. Neuron. 2018. 100(6):1385-1400. e6. (Previewed in the same issue)

10. Zhang KX, Tan L, Pellegrini M, Zipursky SL*, McEwen JM. Rapid Changes in the Translatome during the Conversion of Growth Cones to Synaptic Terminals. Cell Rep. 2016.  14(5):1258-71.

11. Tan L^#, Zhang KX^#, Pecot MY, Nagarkar-Jaiswal S, Lee PT, Takemura SY, McEwen JM, Nern A, Xu S, Tadros W, Chen Z, Zinn K, Bellen HJ, Morey M*, Zipursky SL*. Ig Superfamily Ligand and Receptor Pairs Expressed in Synaptic Partners in Drosophila. Cell. 2015.  163(7):1756-69.