Team's research interests:
(1)Transsynaptic tracing of neural circuits using neurotropic viruses is widely used in defining the synaptic organization of neural networks. Through systematic modification and optimization, we are focusing on the development of rigorous, low toxic and high-brightness transmulti-/monosynaptic HSV1 tracer systems, which can be applied to neural connectome mapping, tumor neural circuit dissection, etc.
(2)Oncolytic virotherapy has become one of the most potential treatments for malignant tumors. Engineered HSV oncolytic virus drugs T-VEC (2015) and G47Δ (2021) were approved for melanoma and glioma treatment in the US and Japan, respectively. We are focusing on the development and quality evaluation of novel HSV oncolytic virus drugs for solid tumors therapy.

Development and quality evaluation of viral vector-mediated neural circuit tracer and oncolytic virus drugs, Neural network mechanism of tumor-nervous system interaction

1. Yusha Sun#, Xin Wang#, Daniel Zhang, Zhijian Zhang, Janardhan Bhattarai, Yingqi Wang, Kristen Park, Weifan Dong, Yun-Fen Hung, Qian Yang, Feng Zhang, Keerthi Rajamani, Shang Mu, Benjamin Kennedy, Yan Hong, Jamie Galanaugh, Abhijeet Sambangi, Sang Hoon Kim, Garrett Wheeler, Tiago Gonçalves, Qing Wang, Daniel Geschwind, Riki Kawaguchi, Angela Viaene, Ingo Helbig, Sudha Kessler, Ahmet Hoke, Huadong Wang, Fuqiang Xu, Zev Binder, H Isaac Chen, Emily Ling-Lin Pai, Sara Stone, MacLean Nasrallah, Kimberly Christian, Marc Fuccillo, Nicolas Toni, Zhuhao Wu, Hwai-Jong Cheng, Donald O'Rourke, Minghong Ma, Guo-Li Ming*, Hongjun Song*. Brain-wide neuronal circuit connectome of human glioblastoma. Nature. 2025 Jan 16. doi: 10.1038/s41586-025-08634-7.
2. Basma Eid Abdullah Ghorab, Tongtan Liu, Min Ying, Ping Wang, Meirong Qin, Jiayong Xing, Huadong Wang*, Fuqiang Xu*. Advances in the Drug Development and Quality Evaluation Principles of Oncolytic Herpes Simplex Virus. Viruses. 2025 Apr 18;17(4):581
3. Yan Chen#, Yuran Song#, Huadong Wang#, Yiyun Zhang, Xinyu Hu, Kaikai Wang, Yingjin Lu, Zoutao Zhang, Shuai Li, Anan Li, Lan Bao, Fuqiang Xu, Changlin Li*, Xu Zhang*. Distinct neural networks derived from galanin-containing nociceptors and neurotensin-expressing pruriceptors. Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2118501119.
4. Min Ying#, Huadong Wang#,*, Tongtan Liu, Zengpeng Han, Kunzhang Lin, Qing Shi, Ning Zheng, Tao Ye, Huinan Gong, Fuqiang Xu*. CLEAR Strategy Inhibited HSV Proliferation Using Viral Vectors Delivered CRISPR-Cas9. Pathogens. 2023 Jun 7;12(6):814.
5. Bing Cai#, Dan Wu#, Hong Xie#, Yan Chen, Huadong Wang, Sen Jin, Yuran Song, Anan Li, Shiqi Huang, Sashuang Wang, Yingjin Lu, Lan Bao, Fuqiang Xu, Hui Gong, Changlin Li*, Xu Zhang*. A direct spino-cortical circuit bypassing the thalamus modulates nociception. Cell Research. 2023 Oct;33(10):775-789.
6. Jin Zhang, Quan Xu, Yifang Zhang, Mohamed F Foda, Kai Cai, Qing Liu, Fan Jia, Huadong Wang, Fuqiang Xu*, Heyou Han*, Huageng Lian*g. Natural bioactive lysosomes extracted from multiple cells for tumor therapy. Biomaterials. 2024:304:122430. 
7. Dennis Jgamadze, James Lim, Zhijian Zhang, James Germi, Kobina Mensah-Brown, Chris Adam, Jiahe Ben Gu, Paul Harary, Rachel Blue, Marissa Fu, Fadi Jacob; Xuyu Qian, Kimberly Gagnon, Matthew Sergison, Oceane Fruchet, Imon Rahaman, Huadong Wang, Fuqiang Xu, Rui Xiao, Diego Contreras, John A. Wolf, Hongjun Song, Guo-li Ming, H. Isaac Chen. Structural and functional integration of human forebrain organoids with the adult rat visual system. Cell Stem Cell. 2023 Feb 2;30(2):137-152.e7. 
8. Xin Liu#, Huadong Wang#, Lin Ma, Min Ying, Qing Liu, Heng Chang, Sen Jin, Fang Yang, Xutao Zhu, Xinghuai Sun*, Fuqiang Xu*, Haixia Liu*. Construction of a mouse model of Posner-Schlossman syndrome by anterior chamber infection with cytomegalovirus. Exp Eye Res. 2022 May;218:109009. 
9. Qing Liu, Yang Wu, Huadong Wang, Fan Jia, Fuqiang Xu*. Viral Tools for Neural Circuit Tracing. Neurosci Bull. 2022 Dec;38(12):1508-1518. 
10. Peng Su#, Min Ying#, Zengpeng Han, Jinjin Xia, Sen Jin, Yingli Li, Huadong Wang*, Fuqiang Xu*. High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy. Molecular brain. 2020; 13(1): 5.

Associate investigator of Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences. I am one of the core researchers in NMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Products, and Shenzhen Key Laboratory of Viral Vectors for Biomedicine. More than 20 invention patents have been applied, and 5 have been authorized. Hosted projects: the Youth Fund and general project of the National Foundation of China, general/key projects in Guangdong and Shenzhen, and participated in the projects of "Brain Science and Brain-like Intelligence Technology-National Science and Technology Major Project", National Key Research and Development Program of China, Strategic Priority Research Program of the Chinese Academy of Sciences, etc. Published/co-published more than 30 SCI papers in Nature, PNAS, Cell research, Cell Stem Cell, Emerging microbes & infections, etc. I’m the editorial board member of Scientific Reports magazine, and the standing committee member of Shenzhen Special Committee for Detection and Evaluation of Cell and Genetic Products.