HUANG Liangyu

HUANG Liangyu

PhD, Associate Investigator
(1) Mechanistic role of liquid-liquid phase separation in synaptic transmission and plasticity; (2) NMR-based metabolomics analysis of neuropsychiatric disorder mouse model.
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Research

Biomolecular condensates or membraneless organelles were discovered to be the hubs for various cellular activities. Even though the proteins or nuclei acids that form a particular condensate are usually known, cellular factors and processes that modulate the transition between phases as well as their respective functions remain to be investigated. Recent studies have found that phase separation of some synaptic proteins plays a role in synaptic plasticity. Hence, with a focus on the active zone formation, we combine super-resolution microscopy and NMR spectroscopy to study the mechanism of ion channel clustering and synaptic vesicles transport at the nanometer to atomic scale, to understand their potential roles in presynaptic plasticity, and to control the phase transition via protein chemistry. Besides, we also plan to setup an NMR-based metabolomics platform for studying different neuropsychiatric disorder mouse models, in hope to develop new diagnostic tool for those disorders.


Biography

2020-present, Associate Investigator at Shenzhen Institute of Advanced Technology, Chinese Academy of Science

2018-2020, Postdoctoral Fellow, University of Toronto
2013-2018, Postdoctoral Fellow, Max Planck Institute for Biophysical Chemistry
2008-2013, School of Biological Sciences, Nanyang Technological University, PhD.
2003-2007, National University of Singapore, BSc in Life Sciences


Selected publications

  1. Wong LE, Kim TH, Rennella E, Vallurupalli P, Kay LE. (2020) Confronting the Invisible: Assignment of Protein 1HNChemical Shifts in Cases of Extreme Broadening. J Phys Chem Lett.11(9):3384-3389.
  2. Wong LE, Kim TH, Muhandiram DR, Forman-Kay JD, Kay LE. (2020) NMR experiments for studies of dilute and condensed protein phases: Application to the phase-separating protein CAPRIN1. J Am Chem Soc.142(5):2471-2489.
  3. Wong LE, Bhatt A, Erdmann PS, Hou Z, Maier J, Pirkuliyeva S, Engelke M, Becker S, Plitzko J, Wienands J, Griesinger C. (2020)Tripartite phase separation of two signal effectors with vesicles priming B cell responsiveness.Nat Commun. 11(1):848.  
  4. Wong LE, Maier J, Wienands J, Becker S, Griesinger C. (2018) Sensitivity-Enhanced Four-Dimensional Amide-Amide Correlation NMR Experiments for Sequential Assignment of Proline-Rich Disordered Proteins. J Am Chem Soc.140(10):3518-3522.
  5. Wong LE, Li Y, Pillay S, Frolova L, Pervushin K. (2012) Selectivity of stop codon recognition in translation termination is modulated by multiple conformations of GTS loop in eRF1. Nucleic Acids Res.40(12):5751-65.